2 de cleanup kit  (GE Healthcare)

Journal: Proteome Science

Article Title: 2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism

doi: 10.1186/1477-5956-7-37

Figure Lengend Snippet: 2-DE gels showing A. baumannii proteins . (A) membrane proteins, (B) cytoplasmatic proteins. Numbered spots (in A) indicate membrane proteins identified by MALDI-TOF/TOF. All gels (12% SDS) were silver stained and loaded with 25 μg total protein.

Article Snippet: Finally, as with the soluble fraction, the extract was processed with a 2-DE Cleanup Kit (GE Healthcare, USA).

Techniques: Staining

Journal: Proteome Science

Article Title: 2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism

doi: 10.1186/1477-5956-7-37

Figure Lengend Snippet: The cytoplasmatic fraction was analysed with IPG strips of various pH range and silver stained gels with different concentrations of acrylamide . 2-DE (12% SDS) gels containing proteins within a range pH 4-7 (A), 2-DE (15% SDS) gel containing proteins within a range pH 3-10 (B), gels were loaded with 25 μg, total protein. Numbered spots indicate proteins identified by MALDI-TOF/TOF.

Article Snippet: Finally, as with the soluble fraction, the extract was processed with a 2-DE Cleanup Kit (GE Healthcare, USA).

Techniques: Staining, SDS-Gel

Journal: Proteome Science

Article Title: 2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism

doi: 10.1186/1477-5956-7-37

Figure Lengend Snippet: 2-DE (12% SDS) gel containing basic proteins within a pH range of 6-11 . Gel was loaded with 40 μg, total protein. Numbered spots indicate proteins identified by MALDI-TOF/TOF.

Article Snippet: Finally, as with the soluble fraction, the extract was processed with a 2-DE Cleanup Kit (GE Healthcare, USA).

Techniques: SDS-Gel